Journal: Cell Transplantation

Article Title: Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy

doi: 10.1177/09636897241226847

Figure Lengend Snippet: Characterization of ADSCs@S-PLGA as cell-laden scaffolds. (A) Schematic illustrations of ADSCs@S-PLGA structure. (B) Scanning electron microscope images depicting PLGA, S-PLGA, ADSCs@PLGA, and ADSCs@S-PLGA. ADSCs were cultured for 24 h on both PLGA and S-PLGA. Scale bars: 50 μm. (C) Optical images showing RFP-ADSCs seeded on the PLGA and S-PLGA scaffold on day 2 of culture. Scale bars: 500 μm. Inset: appearance details of ADSCs@PLGA and ADSCs@S-PLGA. ADSCs: adipose-derived stem cells; S-PLGA: poly(lactic-co-glycolic acid) scaffold; RFP: red fluorescent protein.

Article Snippet: ADSCs were cultured in OriCell TM SD ADSCs growth medium (Cyagen, Santa Clara, CA, USA).

Techniques: Microscopy, Cell Culture, Derivative Assay

Journal: Cell Transplantation

Article Title: Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy

doi: 10.1177/09636897241226847

Figure Lengend Snippet: Characterization of biocompatibility of ADSCs@S-PLGA and differentiative capacity of ADSCs seeded on S-PLGA. (A) Assessment of ADSCs survival following co-culture with PLGA and S-PLGA for 1, 3, 5, and 7 days, determined using the CCK-8 Kit. (B) Visualization of 26SCS on scaffold surfaces using toluidine blue staining assay. (C) Detection of the 26SCS amount immobilized on the scaffold by simulating an in vivo environment. (D) Flow cytometry analysis of CD29, CD34, CD44, CD45, and CD90 expression in ADSCs after coculturing with S-PLGA for 1 day and 7 days. ADSCs: adipose-derived stem cells; S-PLGA: poly(lactic-co-glycolic acid) scaffold; CCK-8: Cell Counting Kit-8; 26SCS: 2-N, 6-O-sulfated chitosan.

Article Snippet: ADSCs were cultured in OriCell TM SD ADSCs growth medium (Cyagen, Santa Clara, CA, USA).

Techniques: Co-Culture Assay, CCK-8 Assay, Staining, In Vivo, Flow Cytometry, Expressing, Derivative Assay, Cell Counting

Journal: Cell Transplantation

Article Title: Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy

doi: 10.1177/09636897241226847

Figure Lengend Snippet: Evaluation of the nanofibrous scaffold in wound healing in vivo . (A) Illustration of the steps taken to establish the ischemic wound healing model. (B) Representative images of full-thickness ischemic skin wound healing process in SD rats with control, S-PLGA, and ADSCs@S-PLGA groups at 0, 7, 14, and 21 days. (C) Wound contraction percentages for the different groups at 7, 14, and 21 days. Statistical significance was defined as */# P < 0.05, **/## P < 0.01, *** / ### P < 0.001. (Significance of the comparison between the control group and ADSCs@S-PLGA group was indicated by * P , and the significance of the comparison between the S-PLGA group and ADSCs@S-PLGAc group was indicated by # P .). SD: Sprague-Dawley; S-PLGA: poly(lactic-co-glycolic acid) scaffold; ADSCs: adipose-derived stem cells.

Article Snippet: ADSCs were cultured in OriCell TM SD ADSCs growth medium (Cyagen, Santa Clara, CA, USA).

Techniques: In Vivo, Control, Comparison, Derivative Assay

Journal: Cell Transplantation

Article Title: Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy

doi: 10.1177/09636897241226847

Figure Lengend Snippet: Histological analysis and investigation of the biochemical characteristics of wound healing after treatment with control, S-PLGA, and ADSCs@S-PLGA. (A) H&E staining of the wound regions to observe wound healing on day 14. The red dotted line indicates the wound surface that did not cover the new epithelia. Scale bar = 1,000 μm. (B) Wound contraction was quantified by measuring the gap between the wound margins. (C) H&E staining of the wound to observe epidermal thickness. Scale bar = 200 μm/100 μm. (D) Quantification of the thickness of the epidermis. (E) Masson staining for evaluation of collagen fibers deposited at the wound sites. Scale bar = 100 μm. (F) Quantification of the collagen density. S-PLGA: poly(lactic-co-glycolic acid) scaffold; ADSCs: adipose-derived stem cells; H&E: hematoxylin and eosin. Statistical significance was defined as * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ADSCs were cultured in OriCell TM SD ADSCs growth medium (Cyagen, Santa Clara, CA, USA).

Techniques: Control, Staining, Derivative Assay

Journal: Cell Transplantation

Article Title: Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy

doi: 10.1177/09636897241226847

Figure Lengend Snippet: Angiogenesis and tissue proliferation assay on wound sections after different treatments. (A) Representative images of immunofluorescent CD31 staining of wound sections in control, S-PLGA, and ADSCs@S-PLGA groups at day 14 after treatment. Endothelial cells were stained with CD31 antibody (red), and the cell nucleus was stained with DAPI (blue) within wound sites. Scale bar = 200 μm/100 μm. (B) Expression of proliferating cell nuclear antigen Ki67 by immunohistochemical staining. Scale bar = 100 μm/50 μm. (C) Quantification of microvessels per microscopic field in different groups. (D) Quantitative analysis of immunohistochemistry. S-PLGA: poly(lactic-co-glycolic acid) scaffold; ADSCs: adipose-derived stem cells. Statistical significance was defined as * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ADSCs were cultured in OriCell TM SD ADSCs growth medium (Cyagen, Santa Clara, CA, USA).

Techniques: Proliferation Assay, Staining, Control, Expressing, Immunohistochemical staining, Immunohistochemistry, Derivative Assay