sd medium Search Results


sd medium  (Valiant Co Ltd)
90
Valiant Co Ltd sd medium
Sd Medium, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pmc03022553-97-5-7?v=Valiant+Co+Ltd
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sd medium - by Bioz Stars, 2026-08
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sd ura medium  (Valiant Co Ltd)
96
Valiant Co Ltd sd ura medium
Sd Ura Medium, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pm25369289-90-28-30?v=Valiant+Co+Ltd
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sd ura medium - by Bioz Stars, 2026-08
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synthetic defined dropout sd medium  (Valiant Co Ltd)
90
Valiant Co Ltd synthetic defined dropout sd medium
Synthetic Defined Dropout Sd Medium, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pm23480849-36-20-25?v=Valiant+Co+Ltd
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synthetic defined dropout sd medium - by Bioz Stars, 2026-08
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sd medium  (Becton Dickinson)
90
Becton Dickinson sd medium
Sd Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pm39526576-398-6-12?v=Becton+Dickinson
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sd medium - by Bioz Stars, 2026-08
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sd-ura medium omnitrays  (Avantor)
90
Avantor sd-ura medium omnitrays
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Sd Ura Medium Omnitrays, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pmc02841618-221-24-29?v=Avantor
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sd-ura medium omnitrays - by Bioz Stars, 2026-08
90/100 stars
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sd-ura agar medium  (Funakoshi ltd)
90
Funakoshi ltd sd-ura agar medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Sd Ura Agar Medium, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/us10066249-207-3-19?v=Funakoshi+ltd
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sd-ura agar medium - by Bioz Stars, 2026-08
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synthetic defined (sd)-ura medium  (US Biological Life Sciences)
90
US Biological Life Sciences synthetic defined (sd)-ura medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Synthetic Defined (Sd) Ura Medium, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pm32687576-107-20-24?v=US+Biological+Life+Sciences
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synthetic defined (sd)-ura medium - by Bioz Stars, 2026-08
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synthetic dextrose medium  (Bufferad Inc)
90
Bufferad Inc synthetic dextrose medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Synthetic Dextrose Medium, supplied by Bufferad Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pmc03355721-81-17-46?v=Bufferad+Inc
Average 90 stars, based on 1 article reviews
synthetic dextrose medium - by Bioz Stars, 2026-08
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modified seawater sd broth medium  (HiMedia Laboratories)
90
HiMedia Laboratories modified seawater sd broth medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Modified Seawater Sd Broth Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pmc11432975-59-7-21?v=HiMedia+Laboratories
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modified seawater sd broth medium - by Bioz Stars, 2026-08
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sd-uwh medium  (Becton Dickinson)
90
Becton Dickinson sd-uwh medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Sd Uwh Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/pmc04655571-498-7-26?v=Becton+Dickinson
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sd-uwh medium - by Bioz Stars, 2026-08
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sd medium  (LiCONiC US Inc)
90
LiCONiC US Inc sd medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Sd Medium, supplied by LiCONiC US Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/bio_rxiv__847095-197-9-15?v=LiCONiC+US+Inc
Average 90 stars, based on 1 article reviews
sd medium - by Bioz Stars, 2026-08
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sd-ura+hygromycin agar medium  (Funakoshi ltd)
90
Funakoshi ltd sd-ura+hygromycin agar medium
(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on <t>SD-URA</t> medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .
Sd Ura+Hygromycin Agar Medium, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sd+medium/us10066249-220-3-19?v=Funakoshi+ltd
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sd-ura+hygromycin agar medium - by Bioz Stars, 2026-08
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Image Search Results


(A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on SD-URA medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .

Journal: PLoS Genetics

Article Title: Multiple Signals Converge on a Differentiation MAPK Pathway

doi: 10.1371/journal.pgen.1000883

Figure Lengend Snippet: (A) Filamentous growth regulation in yeast. At left, different regulatory proteins and pathways that have been implicated in filamentous growth. It is unclear whether RAS regulates the FG pathway, as shown by the question mark. At right, the FG MAPK pathway. Cell surface proteins Msb2p and Sho1p connect to the Rho GTPase Cdc42p and effector p21 activated kinase Ste20p. The MAPKKK Ste11p, MAPKK Ste7p, and MAPK Kss1p regulate the activity of the transcription factors Ste12p and Tec1p. (B) Colonies from the MAT a deletion collection containing p MSB2-HA were pinned to nitrocellulose filters on SD-URA medium and incubated for 48h (left panel). Filters were rinsed in a stream of water and probed with anti-HA antibodies to detect shed Msb2p-HA (right panel). The mutant at position A2 (arrow) was identified as defective for Msb2p-HA secretion. (C) The secretion profile of Msb2p-HA in the haploid ( MAT a) ordered deletion collection. Y-axis, normalized spot intensity (Intensity) as a measure of secreted Msb2p-HA. X-axis, deletion mutants ranked by normalized spot intensity. Yellow, mutants that showed a decrease (2.5 standard deviations below average, - 2.5 SD) in Msb2p-HA secretion. Green, mutants that showed elevated Msb2p-HA secretion (2.5 SD above average). (D) Overlap between genes identified in the deletion screen (blue circle at left, 124 genes) and the overexpression (O/E) screen (yellow circle at right, 389 genes). Seven common genes were identified (see for details). (E) Overlap based on genes that share a common process/function. (F) Heat map showing normalized spot intensity (at 1d and 2d intervals) as a readout of Msb2p secretion compared to expression levels of an MSB2-lacZ ( MZ ) reporter. Yellow, reduced secretion; green, elevated secretion; grey, N/D. See for details. (G) Comparative mucin secretion profiling. Genes that influence Msb2p-HA secretion when overexpressed (∼389 genes) were examined in strains containing Hkr1p-HA (PC2740) and Flo11p-HA (PC2043). Yellow, Msb2p-specific genes; blue, genes common to multiple mucins . (H) Comparison between the secretion profile of Msb2p and regulators of a MAPK pathway growth reporter ( FUS1-HIS3 , ) whose expression is dependent upon the transcription factor Ste12p and elements of the STE pathway , , .

Article Snippet: Transformants were harvested by centrifugation in 96-well plates, resuspended in 30 µl of water, and transferred to synthetic medium containing 2% glucose and lacking uracil (SD-URA) medium in Omnitrays (VWR International Inc. Bridgeport NJ).

Techniques: Activity Assay, Incubation, Mutagenesis, Over Expression, Expressing